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Kalebic Lab

Imaging from the lab

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MOUSE

Mouse brain

Embryonic mouse brain (E15) showing progenitors in magenta. Nuclei are in blue. Acquired by Nereo Kalebic

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FERRET

Ferret postnatal brain

Ferret embryos were electroporated with GFP (green) at embryonic day 37. 20 days later, at postnatal day 16, brain was analyzed and stained by GFAP (magenta). Nuclei are in blue. Acquired by Nereo Kalebic.

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ORGANOIDS

Human cortical organoid

Example of a six-weeks-old human cortical brain organoid stained for progenitor markers SOX2 (green) and Ki67 (magenta) along with DAPI (blue). Acquired by Ilaria Bertani and Stefania Faletti.

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EVOLUTION

Ferret dentate gyrus

Ferret dentate gyrus (postnatal day 16) stained with HOPX (green) and SOX (magenta). Nuclei are in blue. Acquired by Chiara Ossola

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BASAL RADIAL GLIA

Human outer subventricular zone

Basal radial glia in the outer subventricular zone of the human fetal cortex (post conception week 14). The progenitors are marked by HOPX (orange) and SOX2 (magenta). Nuclei are in light blue. Acquired by Emanuele Capra.

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GLIOBLASTOMA

Glioblastoma stem cells in organoid

Glioblastoma stem cells in patient-derived glioblastoma organoids. Nestin (green), SOX2 (red); nuclei (blue). Acquired by Carlotta Barelli.

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GLIOBLASTOMA

GBM cell protrusions

Glioblastoma stem cells growing cell-cell connections that convey resistance to chemotherapy. GFP (green), Phalloidin (white); nuclei (blue). Acquired by Carlotta Barelli.

Image taken by Ilaria Bertani

NEURONS

Mouse cortical neurons and astrocytes

Mouse cortical neurons and astrocytes grown in 2D cell culture. MAP2 (red), GFAP (white), nuclei (cyan). Acquired by Ilaria Bertani.